FastQCFastQC Report
Mon 27 Feb 2023
SRR3127295.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127295.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2651030
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335371.2650554690063862No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119400.086.202521
TAGGGTA7800.073.521214
ATAGGGC15500.071.569463
TAGGGCA15250.071.50984
CGTAGGG9400.071.508162
ATAGCGG6650.069.999051
AGGGATG40950.067.380095
GTACGGG6100.067.040442
ATAGGGA27950.066.766123
AGTAAGG17450.066.55491
ATGCGGG13350.066.546922
TAGAGGG53450.066.484672
TAGGGAT19600.066.431064
GATAGGG41350.065.818882
AGTAGGG42850.065.818482
AGGGCAT23950.065.7486345
TAAGAGG23250.065.7262651
ATAAGGG37350.065.6942442
AGTACGG4100.065.368651
TAAGGGA24200.064.8751753
Produced by FastQC (version 0.11.5)