FastQCFastQC Report
Mon 27 Feb 2023
SRR3127293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334054
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274371.1755083644165902No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99800.085.672131
AGATAGG14600.071.228621
ATAGGGC12500.070.306823
ATAGGGA22450.069.919133
ATAGCGG5850.069.176361
TAGGGCA12850.069.1233144
AGGGATG33450.068.422465
TAGAGGG43400.068.143152
AGTAGGG41350.066.8595052
TAGGGTA7100.066.1923144
TAAGAGG16100.066.053781
AGTAAGG14500.065.878421
ATGAGGG40350.065.25382
TAGCGGG14650.064.508912
TAGGGAT16700.064.444364
AAGAGGG88650.064.440492
GATAGGG37200.064.333352
AAGGGGC66550.064.262734
AGGGAAT27100.063.8181845
AGGGCAT20700.063.7972345
Produced by FastQC (version 0.11.5)