FastQCFastQC Report
Mon 27 Feb 2023
SRR3127292.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127292.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988317
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237881.1963887046180262No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC30300.15239018727899023No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85250.086.710251
ATAGGGC12600.072.002253
GGTAAGG12400.071.672481
TAGGGCA12150.070.0263754
CTACGAC6550.069.61295
CGTAGGG4400.069.441642
GAATAGG11600.069.3186951
TAAGGGA20700.068.352683
AGTAGGG29700.067.740232
TAGCGGG10250.067.414422
AGGGATG25150.066.9120565
AAGGGAC19900.066.8486564
CGAAGGG22250.066.548752
ACGGGAT4100.066.4972154
AGGGTAT10600.066.075395
AGAGGGC33100.065.752513
AGGGCAT16250.065.664685
TAGAGGG40600.065.531442
ATAGCGG3950.065.4753651
ATAGGGA14650.065.456373
Produced by FastQC (version 0.11.5)