FastQCFastQC Report
Mon 27 Feb 2023
SRR3127290.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127290.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811969
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185081.0214302783325764No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27010.14906436037261123No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG26860.14823653164044195Illumina PCR Primer Index 2 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG22290.12301534960035188Illumina PCR Primer Index 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC22220.12262902952533956Illumina PCR Primer Index 2 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT67100.085.281731
TAGGGCG4550.075.395394
TAGGGCA10500.073.398624
ATAGCGG3450.072.2939151
TAAGGGA15900.071.5239643
CGAAGGG17100.071.488852
GACCGAT1250.071.429398
ATAGGGC12400.070.110553
AGTAGGG27650.069.0386052
ATGCGGG9400.069.025942
GTACGGG3350.068.772122
TAGGGTA4750.068.263544
GTAGGGA13300.068.192883
AGGGATG22300.068.0661245
GTAGGGC9950.068.0099953
AAGGGAC16950.067.925014
AGGGTAT8000.067.552475
CGTAGGG3900.067.512552
TGTAGGG13550.066.969822
GGATAGG10350.066.8377761
Produced by FastQC (version 0.11.5)