FastQCFastQC Report
Mon 27 Feb 2023
SRR3127289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2303605
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286751.2447880604530726No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24870.10796121730939116No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT91300.085.589141
TAGGGCA11900.078.208534
CGTAGGG7550.075.331092
ATAGGGC13600.072.92553
TACGGGT1650.071.218353
TAAGGGA22100.069.974453
GAATAGG14600.069.8821
CGAAGGG24100.069.043382
TATAGGG19750.068.304712
AGTAGGG37450.067.650782
AGGGTAT12250.067.532445
TAGAGGG40850.067.428182
AGGGATG30450.067.30315
AGGGAAT26400.066.767215
AATAGGG35600.066.545152
GGTAAGG14600.066.33961
AGGGCAT20150.066.248975
AAGAGGG79650.066.035912
ATAAGGG33450.065.76362
AAGGGGC57300.065.625294
Produced by FastQC (version 0.11.5)