FastQCFastQC Report
Mon 27 Feb 2023
SRR3127287.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127287.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032211
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222711.0958999828265865No Hit
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG26380.1298093554261836No Hit
CTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC26200.12892362062797613TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG24730.12169011977594846No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22140.10894538017951876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT72650.086.488371
CGTAGGG5950.079.021612
TAGGGCA10000.076.60144
TATAGGG17150.070.732412
GAATAGG10850.070.708231
AGGGAAT25500.070.2156455
GGTAAGG11550.070.090411
TAAGGGA17750.069.366863
ATGCGGG9800.069.087472
AGTAGGG32250.068.8137362
TAGAGGG31700.068.6727752
CGAAGGG20400.067.991512
ATAGGGC10600.067.832013
GTACGGG4450.067.621192
ATAGGGA16550.067.297583
AATAGGG29100.067.0529562
AAGAGGG69800.066.8893052
AGGGCAT15150.066.381985
ATAAGGG27250.066.083822
AGTAAGG11300.065.809821
Produced by FastQC (version 0.11.5)