FastQCFastQC Report
Mon 27 Feb 2023
SRR3127286.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127286.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195585
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145931.2205740286136075No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC91340.763977467097697No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC21230.17756997620411763No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG18090.15130668250270787TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC17340.14503360279695712TruSeq Adapter, Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG16660.1393460105304098TruSeq Adapter, Index 13 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12330.10312943036254217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64150.086.936091
CGCTACG14700.084.7271353
ACGACCA14400.084.5339667
GCTACGA14950.083.3102954
GGATGGC37650.082.76427
CGGGTAT1250.082.719275
TCCGCTA15550.082.5551761
AGGGATG44850.082.367165
ATAGCGG1750.080.611171
ATTGCGG2050.080.283481
CGTAGGG3750.080.2126162
GATGGCA37500.079.460638
CTACGAC15700.079.3305055
TAGCGGG4150.079.27642
AAGGGAT32650.079.02844
TAGGGCA6700.077.8649754
TACGACC16000.077.8430566
AGGGATT18100.077.640195
GTAGGGA15150.077.557073
CGAAGGG9650.077.439732
Produced by FastQC (version 0.11.5)