Basic Statistics
| Measure | Value |
|---|---|
| Filename | SRR3127285.fastq |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 1054070 |
| Sequences flagged as poor quality | 0 |
| Sequence length | 100 |
| %GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 11952 | 1.1338905385790319 | No Hit |
| TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC | 7675 | 0.7281300103408692 | No Hit |
| GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG | 2372 | 0.22503249309818135 | TruSeq Adapter, Index 13 (95% over 22bp) |
| CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTG | 2360 | 0.22389404878233893 | TruSeq Adapter, Index 13 (95% over 22bp) |
| CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC | 2208 | 0.20947375411500185 | TruSeq Adapter, Index 8 (95% over 23bp) |
| GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC | 1892 | 0.17949472046448528 | No Hit |
| TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCCAG | 1490 | 0.14135683588376485 | No Hit |
Adapter Content
Kmer Content
| Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| CGCTACG | 1150 | 0.0 | 87.72679 | 3 |
| CGTTTTT | 5000 | 0.0 | 86.87857 | 1 |
| AGGGATG | 3805 | 0.0 | 83.241356 | 5 |
| GGATGGC | 3240 | 0.0 | 83.10812 | 7 |
| GCTACGA | 1210 | 0.0 | 82.9871 | 4 |
| CGAAGGG | 700 | 0.0 | 82.836815 | 2 |
| TCCGCTA | 1225 | 0.0 | 82.78 | 1 |
| CGTAGGG | 280 | 0.0 | 82.50008 | 2 |
| ATTGCGG | 195 | 0.0 | 82.23702 | 1 |
| TCACGGG | 105 | 0.0 | 80.81641 | 2 |
| TAGCGGG | 545 | 0.0 | 80.445694 | 2 |
| CTACGAC | 1265 | 0.0 | 79.86965 | 5 |
| TTGACGG | 65 | 0.0 | 79.81828 | 1 |
| CGGGTAT | 165 | 0.0 | 79.74583 | 5 |
| AGGGAAT | 2105 | 0.0 | 79.47522 | 5 |
| GATGGCA | 3230 | 0.0 | 79.00074 | 8 |
| AAGGGAT | 2830 | 0.0 | 78.79362 | 4 |
| GCGGGTA | 210 | 0.0 | 78.57151 | 4 |
| CGGGATG | 655 | 0.0 | 78.20222 | 5 |
| TACGACC | 1295 | 0.0 | 77.65651 | 6 |