FastQCFastQC Report
Mon 27 Feb 2023
SRR3127283.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127283.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3092139
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348901.1283451358428584No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50290.16263822551314802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110950.084.596241
TAGGGTA8750.074.135674
CGTAGGG7950.071.5442052
ATAGCGG8100.070.2454451
TAGAGGG54350.070.14192
AGTAGGG50450.069.9737242
TAAGGGA28000.069.6700743
ATAGGGC18850.069.075593
TAGGGCA19100.068.909784
GTAGGGT12450.068.716063
ATAGGGA29750.066.835883
GTACGGG7950.066.814012
AGGGTAA13100.066.741795
TATAGGG25950.066.660212
AGGGATG44200.066.5745545
ATAGAGG20700.066.333111
ACTAGGG15000.066.1222152
GGTAAGG18850.065.608811
AGAGGGC43650.065.5826953
TAGCGGG18350.065.578292
Produced by FastQC (version 0.11.5)