FastQCFastQC Report
Mon 27 Feb 2023
SRR3127282.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127282.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2635793
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280901.065713430455275No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42270.1603691943942487No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG30820.11692875730377916No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC26520.10061488136587357Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90100.085.380841
TACGGGT2150.072.349233
CGTAGGG6750.071.228672
TATAGGG20600.070.018482
AGTAGGG42000.069.358162
ATAGGGC14600.068.767813
ATAGCGG5650.068.453641
CTACGAC10950.068.237215
TAAGGGA21100.068.135873
AGGGATG34400.067.894755
TAGGGCA13900.067.822474
GGTAAGG17800.067.304631
GTAGGGT9750.067.19993
ATAGGGA23150.066.3781363
ACTAGGG12150.066.340422
TAGAGGG44600.065.843312
ACGACCA11000.065.790977
AAGAGGG89550.064.849042
CGGGTAT3850.064.692425
CGAAGGG23750.064.304272
Produced by FastQC (version 0.11.5)