FastQCFastQC Report
Mon 27 Feb 2023
SRR3127281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2687447
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271791.011331572306356No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC43120.16044967584477016No Hit
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG31570.11747208410063528No Hit
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC27060.10069035780054453Illumina Single End Adapter 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT89750.084.221861
CGTAGGG7750.072.210962
GTAGGGT11750.071.992183
AGTAGGG43000.071.854632
TATAGGG20900.071.3297652
ATAGGGC14400.070.8186953
TAGACGG3500.069.927671
TAGGGCA12850.069.852334
ATAGCGG5500.069.3164141
TAGTAGG11100.069.115971
GAATAGG18450.068.87811
ATAGGGA23800.068.715223
TACGGGT2150.067.760083
ACTAGGG10600.067.436582
AGTAAGG16900.066.840291
ATAGAGG16850.066.759291
GGTAAGG15700.066.552911
ACGGGTA3500.065.7928544
TAAGGGA23150.065.569553
TAGAGGG47200.065.361152
Produced by FastQC (version 0.11.5)