FastQCFastQC Report
Mon 27 Feb 2023
SRR3127280.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127280.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3371108
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT472861.4026842213301975No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT152550.086.300591
CGTAGGG9750.074.245152
GAATAGG23350.071.48411
AGTAGGG59650.071.080112
AGGGATG53100.070.643675
AGATAGG20250.070.121291
ATAGGGC21200.069.401193
TATAGGG30500.069.198792
TAGGGTA11650.068.190784
GTAGGGT14000.067.152233
ATAGGGA33150.066.78763
ACGGGTA2900.066.458534
AGTACGG6100.066.288261
TAGAGGG60900.066.147822
TGTAGGG25600.066.101992
GATAGGG52250.066.033112
TAGGGCA18050.065.367434
AGTAAGG23950.065.1779251
AAGAGGG123800.065.1171652
ATAAGGG45850.064.998292
Produced by FastQC (version 0.11.5)