FastQCFastQC Report
Mon 27 Feb 2023
SRR3127279.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127279.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884103
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391711.3581692470761273No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT125800.085.2760241
AGGGATG46600.072.10425
CGTAGGG8000.071.869862
AGTAGGG48950.069.704832
ATAGGGA26700.069.367853
ATAGGGC17000.068.196663
TAGGGCA13950.067.56674
CGAAGGG28750.067.5361562
TAGAGGG53050.067.4269262
TATAGGG24500.067.133032
GAGGGAT38350.066.85144
AGAGGGC40300.066.77413
GAATAGG19850.066.753181
GATAGGG43250.066.57822
AGGGAAT27650.066.114225
ATGAGGG48250.065.441682
AATAGGG42900.065.2538452
AAGAGGG103300.065.194182
AGATAGG16450.065.07081
AAGGGGC86300.065.039614
Produced by FastQC (version 0.11.5)