FastQCFastQC Report
Mon 27 Feb 2023
SRR3127278.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127278.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2952261
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT382461.2954816664244795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT124400.087.023941
ATAGGGC16200.073.395093
CGTAGGG8950.073.046452
AGTAGGG50850.071.405832
TATAGGG22350.070.287212
AGGGATG42400.069.2748645
AGATAGG17200.068.1400151
GAATAGG18100.068.1324541
ATAGGGA26100.067.88323
TAGGGTA9500.067.278574
TGTAGGG21000.066.5188142
AGAGGGC44200.066.347393
ATGTAGG10750.066.1149751
GATAGGG46450.065.816522
AGTACGG4800.065.699931
TAGAGGG52200.065.684722
ATAGCGG7100.065.6308751
CGAAGGG27150.065.483112
AAGGGGC86150.065.2980654
ACGGGTA3600.065.272314
Produced by FastQC (version 0.11.5)