FastQCFastQC Report
Mon 27 Feb 2023
SRR3127277.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127277.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709002
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274091.0117748159654367No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28160.10394972022907328No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT97850.082.457471
ATAGCGG8100.073.7211761
AGTAGGG41600.071.5285952
ATAGGGC16600.071.3611453
TAGAGGG53500.071.346322
AAGGGAC31500.069.8400654
TAGTAGG10550.068.634391
GAATAGG16450.068.027521
TAGCGGG17700.067.457432
GGATAGG15850.067.339551
AGAGGGC41050.066.303233
CGTAGGG8100.066.1590352
ATAGAGG20350.065.849731
AGATAGG17550.065.6390841
AGGGACT40850.065.477125
AAGAGGG95550.065.087642
TAGGGCA13150.065.0601354
TAAGGGA22550.065.039533
ATAGGGT11350.065.023923
ATAGGGA24850.064.694753
Produced by FastQC (version 0.11.5)