FastQCFastQC Report
Mon 27 Feb 2023
SRR3127276.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127276.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2335449
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228710.9792977710067742No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT24570.10520460947766361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76950.084.470581
ATAGCGG4650.080.13811
CGTAGGG7250.075.43132
AGTAGGG36100.072.741052
ATAGGGC12400.072.237633
GTAGGGT8500.070.994163
TAGAGGG49200.069.854442
TAGGGCA11150.069.7654954
TAGGGTC7050.069.5465854
GAATAGG14300.069.270471
TAAGGGA18550.069.128413
ATAGAGG20250.068.949571
TAGGGTA7700.067.961644
GGATAGG13100.067.69421
AGATAGG14250.067.527421
ATAGGGA19900.067.0447853
AGTTAGG5700.067.03091
AGGGCAT18750.066.4175
AGTAAGG15450.065.946251
GGTAAGG12250.065.460251
Produced by FastQC (version 0.11.5)