FastQCFastQC Report
Mon 27 Feb 2023
SRR3127274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2199583
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272621.2394167439919292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT87150.084.33391
GAATAGG15400.074.198681
ATAGGGC11750.074.012693
ATAGGGA20100.070.862893
CGTAGGG7100.070.843132
TAGGGCA11700.069.1058654
TAGAGGG40150.069.077852
AAGGGTA16850.068.907964
TATAGGG20400.068.899072
AGGGTAT13450.068.502455
ATAAGGG33000.068.375372
AGTAGGG36850.068.120232
AGATAGG13100.067.8423841
TGTAGGG19700.067.767972
ATGTAGG10350.067.2406541
CGAAGGG21700.066.9377442
AGTAAGG11950.066.894691
AGGGCAT21400.066.5581365
GATAGGG32600.066.474592
TAAGGGA16950.066.282753
Produced by FastQC (version 0.11.5)