FastQCFastQC Report
Mon 27 Feb 2023
SRR3127272.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127272.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974367
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218721.1077980942752792No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG24060.12186184230186181No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTG22580.11436576887680963No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC20750.10509697538502213No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT69850.084.7481461
CGTAGGG5250.083.295272
ATAGGGC10450.071.952743
GAATAGG12150.070.8746261
ATAGAGG13450.069.971911
ACGGGAT4250.068.556154
AGGGATG23950.067.891335
TAAGGGA15550.067.695733
ATAAGGG24950.067.4697652
TAGAGGG34500.067.465672
AAGAGGG69600.067.019192
TATAGGG16050.066.503972
AGTAGGG30050.066.346512
AGTAAGG13200.066.3063351
AGAGGGC28950.066.230083
AAGGGGC56300.066.192454
TAGGGCA9450.066.13994
AGGGCAT17600.065.9519965
TGTAGGG15300.065.4613342
ATAGGGA14800.065.410753
Produced by FastQC (version 0.11.5)