SRR3127270.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3127270.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	908866
Sequences flagged as poor quality	0
Sequence length	100
%GC	43

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11751	1.2929298708500483	No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC	6129	0.674356835881197	No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	2631	0.2894816177522319	RNA PCR Primer, Index 41 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	2579	0.28376020227404264	Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2541	0.279579167886135	RNA PCR Primer, Index 41 (96% over 26bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC	1468	0.16151996003811342	No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG	1122	0.12345054166400767	No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGACATC	1088	0.11970961615903775	No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCT	921	0.10133507029639133	No Hit
AAAGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTT	911	0.10023479808904724	RNA PCR Primer, Index 41 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	4965	89.94969	1
TAAGCGG	95	89.35478	1
ACGGGTA	85	88.73158	4
GCTACGA	1045	85.70664	4
CGGGATG	595	85.298996	5
CGCTACG	1060	84.93851	3
GACCGAT	50	84.588165	8
CTACGAC	1050	84.588165	5
AGGGATG	3040	83.011406	5
CGACCAA	1050	82.797935	8
TCCGCTA	1105	82.795815	1
GATGGCA	2360	82.238495	8
GGATGGC	2515	82.21514	7
ACGACCA	1070	80.81112	7
AGCGGGA	525	80.80911	3
TAACGGG	170	80.412994	2
TAAGGGA	960	80.037506	3
CGTAGGG	165	79.99287	2
CTAACGG	65	79.808334	1
CGAGGGA	610	79.59478	3