FastQCFastQC Report
Mon 27 Feb 2023
SRR3127269.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127269.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931638
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114211.2259053409156775No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC63210.6784824148435337No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTCGACATC29630.3180419862650514No Hit
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC27810.2985065014522808RNA PCR Primer, Index 41 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG27180.29174421824786023RNA PCR Primer, Index 41 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG26580.2853039485293644Illumina PCR Primer Index 11 (95% over 24bp)
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC14730.15810862158907216No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA14510.155747189358957No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCTCG11350.12182843550821242No Hit
TAGACCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCT9850.1057277612119729No Hit
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCT9850.1057277612119729RNA PCR Primer, Index 41 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT48300.088.402971
ACGGGTA1350.087.029484
CGCTACG10300.085.778973
GCTACGA10300.085.322694
AGGGATG25800.085.248415
CGGGATG5750.084.1845
TAAACGG900.083.692051
GTACGGG1700.083.005262
GGATGGC22750.082.217047
TAGCGGG5100.082.082982
CGAGGGA6250.081.9608843
ATAGCGG2250.081.599751
CTACGAC10800.080.9374165
TCCGCTA11000.080.458491
GATGGCA21800.080.410458
AGGGAAT19100.080.212935
TAACGGG2650.079.8729862
GTTACGG650.079.668391
CGACCAA10750.079.565198
AGCGGGA7050.079.326453