FastQCFastQC Report
Mon 27 Feb 2023
SRR3127268.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127268.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871021
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109011.2515197681801014No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC64500.7405102747235716No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC13120.1506278264243916No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG12440.14282089639629814No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG11210.12869953766901143No Hit
TAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATA9950.11423375555813235No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT43300.087.6194461
TAGCGGG3600.083.5732652
AGGGATG25450.082.387615
CGCTACG11150.080.949893
ACGGGTA700.080.593134
CGAAGGG8600.080.354242
GGATGGC18300.079.900437
CTACGAC11300.079.879925
TCCGCTA11250.079.817051
GGGTACG900.078.354446
GCGGGAT5400.077.483834
GCTACGA11650.077.4800954
TAACGGG1950.077.1445542
CGGGTAT800.076.395585
AGGGCAT7950.076.28475
TTACGGG1550.075.822522
GTACGGG2300.075.6247252
ATAGCGG1500.075.220261
TCGTAGG507.094059E-1175.220261
AGTACGG1000.075.220261
Produced by FastQC (version 0.11.5)