FastQCFastQC Report
Mon 27 Feb 2023
SRR3127265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1607972
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190271.1832917488612986No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC35560.22114812944504011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60750.085.075641
AGGGATG30750.076.427395
CGGGTAT2100.073.861625
CGTAGGG4650.072.77862
CTACGAC6850.072.734335
AGAGGGC24300.070.794413
GGATGGC29400.070.664147
ACGACCA7100.070.173267
TAGAGGG26700.069.712082
TAGACGG2500.069.6010061
AGTAGGG27250.069.167492
TACGACC7250.068.7214056
ATAGCGG3300.068.403941
ATAAGGG22000.068.154132
GAGGGAT27000.067.718914
TAAGGGA14500.066.776463
TACGGGT2200.066.2312853
AAGAGGG58350.065.8925862
AGGGTAC9100.065.5973745
ACGGGTA1650.065.519114
Produced by FastQC (version 0.11.5)