FastQCFastQC Report
Mon 27 Feb 2023
SRR3127264.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127264.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386551
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155601.1222089919519729No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31060.22400906998732828No Hit
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG18510.1334967123459577No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC18440.13299186254238035TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG17700.12765487890456248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50200.086.017191
AGGGATG27250.075.880595
TAGACGG2300.073.785011
ACGGGTA1600.073.63564
TAGAGGG22750.072.917052
GTACGGG2500.071.632722
ATAAGGG19150.071.120842
CGGGTAT1600.070.491345
GGATGGC25350.070.4457
TAAGGGA12600.070.3161543
ATAGCGG3100.069.950311
AACGAGG5750.069.6858441
AGAGGGA32550.069.495723
AGGGAAT17650.069.226635
ACGACCA6050.069.1327
AGACGGG9550.069.0863952
GTAGGGA11600.069.065123
AGGGTAC6500.068.6838765
GAGGGAT23100.068.548054
CTACGAC6150.068.007915
Produced by FastQC (version 0.11.5)