FastQCFastQC Report
Mon 27 Feb 2023
SRR3127263.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127263.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417167
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152781.0780663111686908No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC31250.22051035622477805No Hit
CTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC20070.14162057118180144TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG19880.14027986821595478No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG18170.12821354152333495No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50950.084.6954351
CGTAGGG3200.076.4155042
AGGGATG26150.074.763425
ATAAGGG19300.070.659212
TAAGGGA12950.069.315623
AGAGGGC20500.068.546433
GGATGGC24700.068.4972467
ACGGGTA2200.068.3588644
GTAGGGT6900.068.111183
GGTAAGG8050.067.81051
AGGGTAC6350.067.349635
TACGGGT1400.067.138173
GCGATAT353.640509E-667.138178
AACGAGG6450.067.1217041
AGGGTAA6800.067.039445
TAGAGGG24650.066.960442
GAGGGAT21500.066.888354
TACGACC6900.066.748966
AGTAGGG24400.066.6828842
AGTAAGG7950.066.295761
Produced by FastQC (version 0.11.5)