FastQCFastQC Report
Mon 27 Feb 2023
SRR3127261.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127261.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2313381
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252811.0928160990342706No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45220.19547147659637562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80400.084.3978961
ATAGAGG16700.075.673741
CTACGAC9350.071.8729865
TACGACC9300.071.754086
TAGACGG3700.071.369591
CGTAGGG7550.071.163742
AGGGATG40550.071.157195
TAAGAGG16550.070.091281
AGTAGGG41750.069.425582
TAGAGGG47200.069.197772
TAGGGCA12300.068.9712754
TAAGGGA19450.068.817613
TATAGGG18400.068.3902742
ATAGCGG5800.067.480291
AGTAAGG14000.067.364141
AGGGCAT18900.066.885465
AAGAGGG82300.066.830062
GTAGGGA18950.066.654043
ACGACCA10050.066.39937
GAATAGG14150.066.3167951
Produced by FastQC (version 0.11.5)