FastQCFastQC Report
Mon 27 Feb 2023
SRR3127260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2350301
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247521.0531417039774904No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC46210.19661311466063286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT78400.085.450761
ATAGCGG6300.073.182551
AGGGATG38250.072.2461855
CGTAGGG7850.071.871422
TAGGGTA7650.071.2632454
TAGGGCA11850.070.1978764
AGGGTAC11850.069.801285
AGGGCAT18450.069.794835
TAAGGGA17950.069.382583
TATAGGG20250.068.260112
AGTAGGG42500.067.702872
TAGAGGG47700.067.616142
ATAGAGG18100.067.579791
AGTACGG3300.067.004811
GGTAAGG14000.066.5363851
AGGGACT25450.066.478895
CTACGAC9050.066.470735
AAGAGGG84450.066.139582
ATAGGGC13650.065.416913
ATAAGGG29750.065.269122
Produced by FastQC (version 0.11.5)