FastQCFastQC Report
Mon 27 Feb 2023
SRR3127258.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127258.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1932555
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218301.1295926894706747No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68750.084.999031
CGTAGGG6100.074.176032
ATAGGGC10350.073.772913
TAGGGTA6000.073.055674
ATAGCGG4750.072.4844061
AGTAGGG34100.071.459242
TAGGGCA10200.069.7748644
GAATAGG10550.069.293841
GGTAAGG11850.069.254231
AGATAGG10850.068.681961
TAGAGGG35750.068.556632
AGGGTAT10500.068.028945
GTAGGGT7150.067.8974463
AGTAAGG14900.067.739631
GCATAGG7000.066.7040941
AAGGGTA12650.066.693694
AGGGCAT17950.066.498015
ATAGGGA15350.065.709423
TAGCGGG11450.065.450642
CGAAGGG19650.065.0023352
Produced by FastQC (version 0.11.5)