FastQCFastQC Report
Mon 27 Feb 2023
SRR3127256.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127256.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392222
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279621.1688714508937716No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC56430.23588947848485636No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98850.083.9462661
CGTAGGG6800.078.115882
AGGGATG44600.075.465425
ATAGCGG6400.072.7258151
GTACGGG6300.071.630912
TAGCGGG13550.071.465712
TAGGGCA13800.070.511684
GTAGGGT9600.070.022023
TAAGGGA20400.069.589963
AGTACGG3650.069.5559541
TACGGGT2650.069.1812743
GAGGGAT37550.068.852954
AGTAAGG16800.068.563061
GGATGGC43150.068.524957
TAGGGTA9400.068.5113454
AGTAGGG40250.068.438672
TAGAGGG43850.067.965652
AGGGAAT31850.067.8919455
AGGGCAT24350.067.760715
AGGGTAT14650.067.704055
Produced by FastQC (version 0.11.5)