FastQCFastQC Report
Mon 27 Feb 2023
SRR3127255.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127255.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045223
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229261.1209535586095012No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45080.22041606220935323No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG30370.14849236489126125No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC29390.14370071136497095No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG27610.1349975039396682No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT79950.085.066451
CGTAGGG6550.078.44642
AGGGATG39400.077.407995
TAGGGCA11350.075.17454
TAAGGGA17100.074.155613
ATAGCGG4850.073.9066241
TACGGGT1800.073.328593
TAGCGGG10750.072.354122
AGGGTAT12250.070.96975
CGAAGGG20600.070.709722
TCCGCTA9650.070.379591
ATAACGG1550.069.985441
ACGACCA9550.069.875087
AGGGCAT20000.069.785275
AGGGAAT28250.069.533675
GACCGAT950.069.25358
GAGGGAT29800.069.1278464
CTACGAC9800.069.051595
TAGGGAT15450.068.955324
GGATGGC32050.068.62077
Produced by FastQC (version 0.11.5)