FastQCFastQC Report
Mon 27 Feb 2023
SRR3127254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2093795
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222661.06342789050504No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC45890.21917140885330225No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG32540.15541158518384082No Hit
CTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTGC30320.1448088279893686No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTCCATCGTATGCCGTCTTCTG28980.13840896553865112No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23330.11142447087704382No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAGCTCCA23270.11113790987178783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76550.084.659521
CGTAGGG5600.077.269222
AGGGATG39100.076.6827855
ATAACGG1600.076.478681
TAGGGCA12100.074.570974
ATAGCGG5550.072.9277041
TAGCGGG10900.072.923412
GAGGGAT30750.071.677314
TCCGCTA8900.071.3889161
AGTAGGG30500.071.0898742
CTACGAC9000.071.015045
TAGGGTA7500.070.8061754
TAAGGGA17600.070.4928743
AGTACGG2650.069.263721
GTACGGG4650.068.7800752
ATAAGGG28500.068.487282
GTAGGGC11150.068.280093
AGGGTAC8950.068.261255
AGGGTAA10950.068.239685
GGTAAGG12400.067.559331
Produced by FastQC (version 0.11.5)