FastQCFastQC Report
Mon 27 Feb 2023
SRR3127253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3053712
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470671.5413044845093447No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT160500.086.591021
ATAGGGA28450.074.01963
ATAGCGG6350.072.5597461
CGTAGGG8850.071.172552
ATAGGGC15750.071.0309143
GAATAGG21700.070.6319661
ATAACGG3750.070.21021
ACGGGTA4600.069.486764
AGTAGGG51450.069.161032
AGTAAGG18550.068.432641
ATAGAGG22300.068.309891
AATAGGG47950.068.131382
ACTAGGG14400.067.8971942
TAGAGGG54250.067.8442462
AGATAGG22600.067.819191
TAGGGCA15250.067.811584
ATAAGGG44900.067.420272
AGGGATG45800.067.327015
TAAGGGA26600.067.151073
TAGCGGG18600.066.7178342
Produced by FastQC (version 0.11.5)