FastQCFastQC Report
Mon 27 Feb 2023
SRR3127252.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127252.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648798
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387761.4639092901761477No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC27050.10212179260177635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT126500.087.473281
ATAGGGA21950.074.099763
GAATAGG17450.073.25071
ATAGGGC14050.073.149683
ATAGCGG5700.072.8191761
AGTAAGG16800.071.311961
TAAGGGA21700.069.73933
AGATAGG16300.069.737661
ACGGGTA3600.069.407384
AATAGGG38550.068.974262
AGGGCAT22500.068.923585
AGGGATG38700.068.3655
TAGAGGG47000.068.209262
GTAGGGA15800.068.03153
TAGGGCA13450.068.000444
ATAAGGG36100.067.909172
AAGAGGG91000.067.556712
AGTAGGG42400.067.492442
AGGGAAT26450.067.33645
GGTAAGG15000.066.97711
Produced by FastQC (version 0.11.5)