FastQCFastQC Report
Mon 27 Feb 2023
SRR3127251.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127251.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705151
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376831.393009114833146No Hit
CTGTCTCTTATACACATCTGACGCCCTACGCATCGTATGCCGTCTTCTGC28300.10461523219960733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119700.087.13261
AGGGATG38000.072.599395
ATAGGGC15750.072.212653
ATAACGG2800.070.598631
ATAGGGA21050.070.552673
AGTAAGG16550.070.527541
CGTAGGG9300.069.782682
AGTAGGG44050.067.0448152
GGAGCTA45250.066.887629
GAATAGG17100.066.607681
TGTAGGG18100.066.513972
GGTAAGG16600.066.34571
TAGAGGG46200.066.265952
GAGGGAT30400.066.16814
TAACGGG10250.066.067842
ATAGAGG17700.065.679341
CGAAGGG25500.065.6540152
AAGAGGG96250.065.325422
ATAGCGG5550.065.298441
TAGGGAT14200.065.201324
Produced by FastQC (version 0.11.5)