FastQCFastQC Report
Mon 27 Feb 2023
SRR3127250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1855335
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194001.0456332683854936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT66200.083.32651
CGTAGGG5850.073.116922
AGTAGGG31850.071.87072
ATAGGGA19400.070.021033
AGGGATG25250.069.993685
TAGGGCA11000.069.650994
GGTAAGG12050.069.46661
ATAGGGC11950.068.8339163
ATAGCGG4400.068.402251
AAGAGGG69500.067.9017942
GAATAGG13950.067.421581
TATAGGG18350.067.1115952
GATAGGG28000.065.97312
TAGAGGG34150.065.92912
GACCGAT2300.065.4000248
TAGGGTC6400.065.364574
AGAGGGC26800.064.893213
GTAGGGT9200.064.885593
TAGTAGG8200.064.804881
AGGGACT20800.064.6301355
Produced by FastQC (version 0.11.5)