FastQCFastQC Report
Mon 27 Feb 2023
SRR3127249.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127249.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627717
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155990.9583361235399028No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT54200.083.622511
CGTAGGG4250.076.537072
GACCGAT2000.075.1891868
TAGGGCA8900.072.037834
ATAGGGC8200.070.71363
ATAGGGA14100.069.5433963
AGTAGGG26100.068.997692
GGATAGG10250.068.5585251
TAGAGGG28550.068.0303652
GTAGGGC6250.067.8850563
TAGTAGG7100.067.754931
AGAGGGC21500.066.876433
AAGAGGG62350.066.763012
AGGGATG23550.066.24955
TAGGGTC4000.065.999364
GTAGGGT6300.065.84973
ATAGAGG12300.065.5677261
ATAGCGG3900.065.302261
GAGGGAT19350.065.0491944
AAGGGGC45150.064.005074
Produced by FastQC (version 0.11.5)