FastQCFastQC Report
Mon 27 Feb 2023
SRR3127248.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127248.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1645978
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156560.951167026533769No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55150.083.284951
CGTAGGG4600.077.679892
GACCGAT950.074.2031868
ATAGGGA14250.071.564853
ATAGAGG11650.070.692661
ATAGGGC9650.070.614783
AGTAGGG27150.068.576952
TAGGGAT9650.068.179794
ATAACGG1450.068.157481
AGAGGGC24000.067.7516253
AAGGGTA12400.067.461074
GGATAGG10050.067.430851
GGTAAGG8550.067.1515351
TAGAGGG28150.067.142962
GTAGGGT5050.067.003273
AAGAGGG64300.066.832552
TAGGGCA9450.066.141614
AGATAGG9450.065.736161
CGAAGGG18800.065.273292
TATAGGG14700.064.927932
Produced by FastQC (version 0.11.5)