FastQCFastQC Report
Mon 27 Feb 2023
SRR3127247.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127247.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3265128
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400191.2256487341384472No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32810.10048610651711051No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT139300.085.234291
TAGGGCA18550.074.4974064
AGTAAGG20250.074.074681
ATAGGGC21700.071.697843
AGGGATG48350.071.0654755
CGTAGGG8500.070.229992
AGTAGGG55150.068.9510352
ACGGGTA4050.068.4754264
TAGGGTA9800.067.628674
TAAGGGA28900.067.334913
AATGCGG9450.067.174751
TAGAGGG58200.067.114422
GAATAGG22500.066.66721
ATAGGGA30300.066.550673
ATAGCGG6650.066.467641
AGGGCAT28750.066.378275
ATAGAGG23150.065.40471
AGGGTAT19550.064.916455
AAGGGTA22550.064.617914
ATAAGGG47650.064.612442
Produced by FastQC (version 0.11.5)