FastQCFastQC Report
Mon 27 Feb 2023
SRR3127244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419964
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303841.2555558677732397No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC52850.21839167855389582No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC26600.10991899053043763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT98600.083.3997041
AGGGATG55900.075.767655
TAAGGGA20800.073.449843
ACGGGTA2500.073.332324
AGTAGGG38750.073.271672
AGGGCAT23800.073.07955
TAGGGCA14850.072.806844
GTACGGG5850.072.319842
ATAGGGC15900.071.8422553
GGATGGC46100.070.97077
GGTAAGG13800.070.184261
ATAGGGA25400.069.956633
AGGGAAT27700.069.408775
GAGGGAT41550.068.899664
AGGGATC22750.068.807155
AGATAGG14150.068.7805251
AAGGGAT51650.068.5323264
TAGAGGG45300.068.384562
CGTAGGG6550.068.179392
TGTAGGG19100.067.6815262
Produced by FastQC (version 0.11.5)