FastQCFastQC Report
Mon 27 Feb 2023
SRR3127242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096049
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238001.1354696383529201No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC44880.21411713180369352No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC29030.13849867059405577No Hit
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG26830.12800273276054136No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTG26600.1269054301688558No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC22850.10901462704354717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT76800.082.928751
AGGGATG45900.077.200855
TAGGGCA11100.075.786794
AGTAAGG12300.074.2446441
ACGGGTA2750.073.485094
TAGGGCG4400.072.630614
ATAGGGC12300.072.595883
AGTAGGG34200.072.2140352
ATAGCGG4900.071.089291
AAGGGAT41600.070.8333364
AGATAGG11100.070.8211
ATAGGGA19750.070.672893
GGATGGC40150.070.582217
TAAGGGA19600.070.254653
CGAAGGG22100.069.818422
TATAGGG15850.069.450452
TGTAGGG16250.068.898862
GGTAAGG12300.068.886791
TAGGGAT19100.068.649024
GTAGGGA18900.068.6294863
Produced by FastQC (version 0.11.5)