FastQCFastQC Report
Mon 27 Feb 2023
SRR3127240.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127240.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334278
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328941.409172343654012No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27920.11960871841314531No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT107800.087.364961
ATAGGGC13950.073.9664153
TAGGGCA11650.073.605484
TAGAGGG37750.072.763952
AGGGATG35600.071.415775
GAGGGAT29400.069.711854
AGTAGGG40050.069.644022
AGAGGGC34650.069.211653
GGTAAGG13200.068.9197851
ATAGAGG15300.067.778471
AGGGCAT21750.066.548515
CGAAGGG27050.065.6657262
GCATAGG6750.065.6424261
AGATAGG13900.065.449011
ATAAGGG30800.065.3193362
AGTAAGG15450.065.289881
AGGGAAT24050.065.264695
ATAGCGG5450.064.8672261
TATAGGG20550.064.654972
TAAGGGA18250.064.541913