FastQCFastQC Report
Mon 27 Feb 2023
SRR3127239.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127239.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2407709
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315691.3111634337870566No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC28100.11670845604680631No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106150.085.934961
CGTAGGG6950.072.399882
ATAGGGC13900.071.6785663
AGGGATG35850.070.921245
AGTAGGG41450.070.2271352
TAGGGCA12350.070.019514
TAGAGGG39300.070.000662
ATAGGGA20100.068.975393
GAATAGG15150.067.108451
GGTAAGG13600.065.758341
TAGGGTC6150.065.719094
TATAGGG18650.065.559162
GAGGGAT29900.064.91534
GATAGGG35250.064.835972
AAGAGGG89700.064.588792
AGAGGGC34900.064.502763
TAGTAGG11550.064.388931
AGTAAGG15950.064.3327261
TAGGGAT13450.064.293014
TAGGGTA5650.064.048734
Produced by FastQC (version 0.11.5)