FastQCFastQC Report
Mon 27 Feb 2023
SRR3127238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3127298
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394721.2621758463696136No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC61890.19790247043933773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT138300.083.906851
AGGGATG52200.073.122135
CGTAGGG8900.072.887392
TAGACGG3250.070.890361
CTACGAC12700.070.325565
GTACGGG7300.070.188662
AGTAGGG50450.069.881692
TAGAGGG64100.068.493942
TAGCGGG19300.068.196812
ATAGCGG10150.068.096651
ATAGGGA31450.068.007073
TAAGGGA28650.067.270173
CGAAGGG29700.067.266032
TATAGGG26550.066.748292
ATGCGGG16550.066.179162
ATAGAGG22900.065.908891
ACGACCA13200.065.8821957
TAGGGCA17300.065.755574
ATAAGGG43700.065.723872
ACGGGTA3300.065.5250244
Produced by FastQC (version 0.11.5)