FastQCFastQC Report
Mon 27 Feb 2023
SRR3127237.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127237.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2694596
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT326191.2105339724396533No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC51770.1921252759226244No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC31370.11641819404467312No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG29130.1081052595639569No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT116300.085.997351
AGGGATG45750.072.827255
TAGAGGG44650.071.147632
AGTAGGG41550.070.103472
TAGACGG2650.069.397351
CGAAGGG25350.068.979172
CTACGAC12350.068.492715
CGTAGGG6850.068.118752
TAGGGCA14200.068.043644
ATAGGGA23800.067.926493
ACGGGAT11050.067.8198554
TAAGAGG21200.067.39551
GAGGGAT40950.067.332394
ATAAGGG36400.067.332382
GGTAAGG17550.067.171811
GTAAAGG22700.067.096651
ATAGAGG17750.066.946261
AGGGACT32550.066.844985
GTACGGG6350.066.80222
TAAGGGA23800.066.540243
Produced by FastQC (version 0.11.5)