FastQCFastQC Report
Mon 27 Feb 2023
SRR3127234.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127234.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503339
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61461.2210458557751336No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC36910.733303002548978No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC29210.5803245923721388No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG27780.5519143161964402No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG23600.46886889352901323No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA10870.215957833587304No Hit
GAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT9840.19549448781040216No Hit
AGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT8530.1694682907543425No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTAGACG8260.16410411273515463No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA8120.16132268709557576No Hit
CGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT7850.1559585090763879No Hit
TCCGCTACGACCAACTCATACACCTGTCTCTTATACACATCTGACGCATT7050.14006464827879422No Hit
AAAGCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT6560.1303296585402681No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCC6260.12436946074117046No Hit
ACTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG6230.1237734409612607No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCATTAGACGTCG5800.11523049078255411No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG5600.11125702558315569No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG5570.11066100580324593No Hit
AAATCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTT5390.10708488712378735No Hit
TAGACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCT5290.10509815452408813No Hit
GGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCC5100.10132336258465964No Hit
ACCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCT5080.10092601606471979No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT156.8430055E-494.196133
CGGGTAT201.8378334E-593.990265
CGGTTAT156.902782E-493.990265
CGTTTTT34150.088.49181
CGCTACG5750.086.824273
GGATCCC600.086.157747
CGAAGGG4200.084.10372
AGGGATG11300.084.0095
GCTACGA6000.083.206594
ACGCGGG1700.083.114242
AGACGGG2750.082.207542
GGATGGC9550.081.687877
GGGCGAT750.081.458236
CTACGAC6200.081.10455
AGGGCAT3550.080.75225
ACGGGGC1350.080.241154
ACGACCA6100.080.122857
TCCGCTA6450.078.9404751
ACCGGGT301.4370235E-678.496773
GATGGCA9550.078.24328
Produced by FastQC (version 0.11.5)