FastQCFastQC Report
Mon 27 Feb 2023
SRR3127232.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127232.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10388969
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4422494.2569094199819055No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT429800.4137080397486988No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT267270.25726325682558104No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT243730.2346046080222205No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT228770.22020471906307545No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT170810.1644147749406125No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT161010.1549816926010656No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTC124240.11958838263931677No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG116600.11223442865215981No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114450.1101649258939939No Hit
CGTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC111850.10766227139574677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1210350.090.818921
AGGGACT228150.069.7251365
GACTTAA178300.069.266818
GGATAGG53400.068.9306951
AGTAGGG145650.068.719992
GTTTTTT1623850.067.9176942
ATAGGGC44800.067.917693
ATAGGGA60250.067.62193
ACTTAAT182650.067.462779
TAGTAGG32250.067.225041
AGATAGG52250.066.659341
GGACTTA187450.066.562597
GATAGGG138450.066.340132
GGTAAGG56750.066.27351
ATAGAGG67300.065.8987961
AAGGGGC353800.065.7254644
GTAGGGA66850.065.173153
AATAGGG125550.064.2651142
TAGGGAC42900.063.8994
AGTAAGG62300.063.773821