FastQCFastQC Report
Mon 27 Feb 2023
SRR3127231.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127231.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6976889
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2646633.7934242611570856No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT262960.3769015101143217No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT257130.3685453502270138No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT249170.35713625370849383No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT216970.3109838783446318No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC177260.25406739307447773No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTC121910.17473403977044782No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG108830.1559864289083573No Hit
CGTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC108410.15538444140361127No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT100860.14456299935401007No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT93960.1346732046331825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT768300.090.82381
AGGGACT139350.071.834845
GACTTAA113600.069.996818
ATAGGGC28600.069.5093153
ACTTAAT113850.069.4715969
GGATAGG34100.069.231611
TAGTAGG20000.068.425521
GTTTTTT1027700.067.9503862
TAGGGAC26200.067.804124
AGTAGGG95100.067.554882
GGACTTA119850.067.367067
AAGGGGC233600.066.2699054
AGATAGG33750.065.908661
GGTAAGG35450.065.7991941
GATAGGG88150.064.937222
AATAGGG78900.064.330282
AGTAAGG40750.064.281011
CAGGGAC132450.063.7619674
ATAGAGG43600.063.7464681
ATAGGGA37050.063.6772353