FastQCFastQC Report
Mon 27 Feb 2023
SRR3127230.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127230.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10402649
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4169394.008007960280117No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT432000.41527883907262464No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT265790.25550222832665026No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT247670.23808358813221514No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT242420.233036796685152No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT169990.16341030058785988No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT163050.15673892294164687No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC127350.12242074110161748No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTC124450.1196329896356207No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG117710.11315387071120057No Hit
CGTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC114150.10973166546328728No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108000.10381970976815616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1178400.090.535061
ATAGGGC47300.070.3462753
AGGGACT232300.069.959195
GACTTAA186400.068.881698
ACTTAAT188500.067.690469
GTTTTTT1600000.066.9724042
GGTAAGG56100.066.588731
GGATAGG55400.066.495931
GGACTTA195050.066.429317
AGATAGG53450.066.2812041
ATAGGGA57700.066.137723
AGTAGGG145400.065.903922
TAGGGAC43600.065.8603064
AAGGGGC348200.065.4069064
ATAGAGG65950.065.346721
GATAGGG138500.065.3170852
AATAGGG125450.064.953032
GTATAGG28350.064.556451
GTAGGGA63450.063.9957473
TATAGGG69400.063.8209042
Produced by FastQC (version 0.11.5)