FastQCFastQC Report
Mon 27 Feb 2023
SRR3127229.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127229.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12352741
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5200994.210393466518888No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT526100.4258973777560786No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCT294100.23808481048861949No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT265340.21480252844287756No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT231990.18780447189818034No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT210410.17033466499459513No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT199140.16121118381742158No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG147670.11954431813959347No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145280.11760952488196749No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTC141100.11422566052344171No Hit
CGTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC127310.10306214628801819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1424950.090.588991
ATAGGGC55200.071.631373
AGGGACT281900.071.482535
GACTTAA227100.070.149278
ACTTAAT228700.069.1653449
ATAGAGG81600.068.503411
GGACTTA235100.068.461837
AGTAGGG168950.068.073772
GGTAAGG62500.067.831381
GTTTTTT1923350.067.473342
ATAGGGA74550.067.228883
GGATAGG64500.067.1141361
AGATAGG59600.066.947671
AAGGGGC420150.066.1802754
TAGGGAC52850.065.476344
GATAGGG161950.065.209422
GTATAGG35500.065.078731
GTAGGGA80150.064.6434253
TATAGGG81600.064.248982
AATAGGG158250.064.178792