FastQCFastQC Report
Mon 27 Feb 2023
SRR3127228.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127228.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11463089
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4560773.9786570618094306No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT508990.4440251663404166No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT247030.21550037690538734No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT242070.21117344548227793No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT242050.21115599817815248No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT211480.1844877938224156No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG198350.1730336386640634TruSeq Adapter, Index 22 (95% over 21bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT180020.15704318443309653No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC129980.11339002951124255No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126850.11065952641561101No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC122420.10679494855182578No Hit
CGTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC121450.10594875430174189No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA118580.10344506615974107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1304850.090.532631
AGGGACT269000.071.453775
GACTTAA219950.070.530298
GGATAGG57650.070.2899551
ATAGGGC52400.070.0972753
ACTTAAT223450.069.236269
AGTAGGG159750.068.596932
GGACTTA230750.067.8198247
GGTAAGG60000.067.615461
ATAGAGG73800.067.166621
ATAGGGA72350.066.714943
AGATAGG56850.066.637651
GTTTTTT1801300.065.860612
AAGGGGC385200.065.832024
TAGGGAC49950.065.710554
GATAGGG150550.065.250332
TATAGGG75050.064.692982
GTAGGGA73350.064.6497963
AATAGGG135200.064.403492
ATAAGGG131050.064.250972
Produced by FastQC (version 0.11.5)