FastQCFastQC Report
Mon 27 Feb 2023
SRR3127226.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3127226.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11560624
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4302263.721477318179365No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT518080.4481418996068032No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT258950.22399309933443037No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT243430.21056821846294804No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT241830.2091842101256818No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT215020.18599342042436465No Hit
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG204560.1769454659194867TruSeq Adapter, Index 22 (95% over 21bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT184760.15981836274581718No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC152800.13217279620892436No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC135820.11748500772968656No Hit
CGTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC127340.11014976354217557No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC121580.10516733352801719No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117980.10205331476916817No Hit
GAGCAGGAGGCTGACAATGAGGTAGACGAAGAAGAGGAAGAAGGTGGGGA117650.101767863049607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1274750.090.368131
AGGGACT274550.071.656435
ATAGGGC50850.071.2591863
GACTTAA224650.070.18818
ACTTAAT228500.068.717559
AGTAGGG161300.068.383812
GGACTTA234350.067.964797
TAGGGAC52800.067.559314
GGTAAGG62950.067.4251561
ATAGAGG73050.067.3142851
ATAGGGA67900.067.070483
TATAGGG72550.066.297612
GTATAGG33200.066.189641
GGATAGG60550.065.978841
GTTTTTT1768750.065.512172
GTAGGGA74500.065.418393
GATAGGG154100.064.988512
AAGGGGC386650.064.920524
AGATAGG59000.064.601741
TAGTAGG39400.064.372991
Produced by FastQC (version 0.11.5)